In one trial, young men drank a litre of black coffee with 212 mg of caffeine and passed no more urine over four hours than on water. Tea and cola, with less caffeine, did no worse.
With 17 men on coffee, the trial was sized to catch a difference of 220 ml of urine or more, so a smaller loss could have gone unseen. Larger doses were not tested, and the researchers note a measurable effect on urine is likely once a single dose passes about 300 mg of caffeine. The men were fully hydrated and had fasted overnight. The litre went down in half an hour, not as cups spread across a day.
